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rabbit anti runx2 polyclonal ab  (Proteintech)


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    Structured Review

    Proteintech rabbit anti runx2 polyclonal ab
    (A) Western blots revealing <t>Runx2</t> and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.
    Rabbit Anti Runx2 Polyclonal Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+runx2+polyclonal+ab/Animal-free+Recombinant+Human+Wnt3A/pmc11911064-132-42-48
    Average 95 stars, based on 102 article reviews
    rabbit anti runx2 polyclonal ab - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament"

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament

    Journal: JBMR Plus

    doi: 10.1093/jbmrpl/ziaf021

    (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.
    Figure Legend Snippet: (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

    Techniques Used: Western Blot, Expressing, Derivative Assay, Control, Cell Culture

    Related Articles

    Incubation:

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament
    Article Snippet: The total protein (10 μg per well) was separated on 12.5% SDS-PAGE and transferred onto polyvinylidene difluoride membrane (PE Applied Biosystems) for 70 min. .. The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins. .. After washing 3 times in 0.1 M PBS, the membranes were incubated for 1 hr in anti-rabbit secondary IgG/ HRP complex (dilution, 1:5000) (Santa Cruz Biotechnology).

    Quantitative Proteomics:

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament
    Article Snippet: The total protein (10 μg per well) was separated on 12.5% SDS-PAGE and transferred onto polyvinylidene difluoride membrane (PE Applied Biosystems) for 70 min. .. The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins. .. After washing 3 times in 0.1 M PBS, the membranes were incubated for 1 hr in anti-rabbit secondary IgG/ HRP complex (dilution, 1:5000) (Santa Cruz Biotechnology).



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    rabbit anti runx2 polyclonal ab  (Proteintech)
    95
    Proteintech rabbit anti runx2 polyclonal ab
    (A) Western blots revealing <t>Runx2</t> and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.
    Rabbit Anti Runx2 Polyclonal Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+runx2+polyclonal+ab/Animal-free+Recombinant+Human+Wnt3A/pmc11911064-132-42-48
    Average 95 stars, based on 1 article reviews
    rabbit anti runx2 polyclonal ab - by Bioz Stars, 2026-10
    95/100 stars
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    rabbit anti rat runx2  (Elabscience Biotechnology)
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    Elabscience Biotechnology rabbit anti rat runx2
    (A) Representative images of safranin O staining, TNMD, and <t>RUNX2</t> immunohistochemistry in the cartilage tissue. Original magnification: 40×. (B) Percentage area of TNMD histological analysis for each group. (C) Percentage area of RUNX2 histological analysis for each group. Each bar displays the mean value (standard deviation, n = 5). *p < 0.05. RC: rotator cuff repair; S-hMSC: secretome-hypoxia mesenchymal stem cells
    Rabbit Anti Rat Runx2, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal anti runx2 cbfa1 ab  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology rabbit polyclonal anti runx2 cbfa1 ab
    (A) Representative images of safranin O staining, TNMD, and <t>RUNX2</t> immunohistochemistry in the cartilage tissue. Original magnification: 40×. (B) Percentage area of TNMD histological analysis for each group. (C) Percentage area of RUNX2 histological analysis for each group. Each bar displays the mean value (standard deviation, n = 5). *p < 0.05. RC: rotator cuff repair; S-hMSC: secretome-hypoxia mesenchymal stem cells
    Rabbit Polyclonal Anti Runx2 Cbfa1 Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+runx2+polyclonal+ab/ICAM-1+Antibody/pm16208410-70-20-24
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    Image Search Results


    (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

    Journal: JBMR Plus

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament

    doi: 10.1093/jbmrpl/ziaf021

    Figure Lengend Snippet: (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

    Article Snippet: The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins.

    Techniques: Western Blot, Expressing, Derivative Assay, Control, Cell Culture

    (A) Representative images of safranin O staining, TNMD, and RUNX2 immunohistochemistry in the cartilage tissue. Original magnification: 40×. (B) Percentage area of TNMD histological analysis for each group. (C) Percentage area of RUNX2 histological analysis for each group. Each bar displays the mean value (standard deviation, n = 5). *p < 0.05. RC: rotator cuff repair; S-hMSC: secretome-hypoxia mesenchymal stem cells

    Journal: Archives of Bone and Joint Surgery

    Article Title: Combination Effect of Rotator Cuff Repair with Secretome-hypoxia MSCs Ameliorates TNMD, RUNX2, and Healing Histology Score in Rotator Cuff Tear Rats

    doi: 10.22038/ABJS.2023.67933.3218

    Figure Lengend Snippet: (A) Representative images of safranin O staining, TNMD, and RUNX2 immunohistochemistry in the cartilage tissue. Original magnification: 40×. (B) Percentage area of TNMD histological analysis for each group. (C) Percentage area of RUNX2 histological analysis for each group. Each bar displays the mean value (standard deviation, n = 5). *p < 0.05. RC: rotator cuff repair; S-hMSC: secretome-hypoxia mesenchymal stem cells

    Article Snippet: Paraffin-embedded sections (5 μm) were then deparaffinized and rehydrated, fully washed, antigen retrieval, blocked for 30 minutes with Blocking Buffer (StarrTrek, Biocare Medical, CA, USA) and overnight-incubated using a rabbit-anti-rat RUNX2 (1:200 dilution; Elabscience Cat. No. E-AB-53343) and TNMD polyclonal antibody (1:200 dilution; ABclonal Cat No. A2851) in the humid chamber.

    Techniques: Staining, Immunohistochemistry, Standard Deviation